Enzymatic Synthesis Of DNA Assignment Help

This method uses the bacterial enzyme polynucleotide phosphorylase, which is specific for ribonucleotides, but polymerizes deoxyribonucleosides albeit at a slow rate when Mn2+ replaces Mg2+ in the reaction mixture. It uses as substrate deoxyribonucleosides diphosphates and does not require a template. But it does need a primer at least 3 nucleotides long. The enzyme adds one or two nucleotides at a time to the primer (and to the growing chain).

The main topics which are considered in Recombinant DNA Technology are discussed in brief as follows:

1. Gene Cloning
2. Vector
3. Chemical Synthesis Of Gene
4. Enzymatic Synthesis Of DNA
5. Polymerase Chain Reaction

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